Esdeveniments

Carregant Esdeveniments

« Tots els Esdeveniments

abril 11 @ 12:00 - 13:30

Interaction dynamics of disordered proteins from single-molecule spectroscopy

Speaker: Prof. Ben Schuler, PhD – Department of Biochemistry, University of Zurich, Switzerland.

Abstract

The functions of proteins have traditionally been linked to their folded structures, but many proteins perform essential functions without being folded. Quantifying the highly dynamic and conformationally diverse ensembles of these intrinsically disordered proteins (IDPs) and their interaction mechanisms is an important aspect of understanding their functions. A remarkable example are highly charged IDPs, which can form high-affinity interactions but retain their disorder in the complexes. I will illustrate how advanced single-molecule spectroscopy combined with molecular simulations and other biophysical methods can be used to probe the dynamics, interactions, and phase separation of such disordered systems.

References:
Borgia et al. (2018) Extreme disorder in an ultrahigh-affinity protein complex. Nature 555, 61-66
Sottini et al. (2020) Polyelectrolyte interactions enable rapid association and dissociation in high-affinity disordered protein complexes. Nat. Commun. 11, 5736
Heidarsson et al. (2022) Release of linker histone from the nucleosome driven by polyelectrolyte competition with a disordered protein. Nat. Chem. 14, 224-231
Chowdhury et al. (2023) Driving forces of the complex formation between highly charged disordered proteins. Proc. Natl. Acad. Sci. USA 120, e2304036120
Galvanetto, Ivanović et al. (2023) Extreme dynamics in a biomolecular condensate. Nature 619, 876-883.

Organitzador

IRB Barcelona – Institut de Recerca Biomedica
Visualitza el lloc web de Organitzador

Recinte

Edifici Cluster I, Aula Fèlix Serratosa
C/ Baldiri Reixac 10-12
Barcelona, Barcelona 08028 Espanya
+ Mapa de Google
Telèfon
934037171
Visualitza el lloc web de Recinte